Real Time quantitative PCR High risk Human Papillomavirus ( HPV ) Nucleic Acid DNA Detection Kit
Intended use:
This kit, adopting Real-Time PCR-Fluorescence technology, is used for qualitative detection of HPV 16+ 18. It can be used for the laboratory diagnosis and monitoring of the virus infection. The test results are only for clinical reference, not for case confirmation or exclusion.
Test Principle:
The specific primers and specific fluorescent probes are designed for the conserved region of HPV 16+ 18. The PCR reaction solution, and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of HPV 16+ 18 through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of the nucleic acid extraction is qualified.
Product components:
| No. |
Components |
BIK-QL-H005 |
| 1 |
PCR Enzyme Mix (Lyophilized) |
96 tests/bottle |
| 2 |
HPV Primer-Probe Mix |
100 μL/vial |
| 3 |
Enzyme Mix Buffer (5×) |
400 μL/vial |
| 4 |
Positive Control |
100 μL/tube |
| 5 |
Negative Control |
100 μL/tube |
| 6 |
RNase-free water |
1mL/tube |
| 7 |
Paraffin oil |
1.5mL/tube*2 |
Note: Do not mix the components from different batches for detection. The Positive Control of HPV and internal control were constructed artificially, and they were not infectious.
Data anlysis:
The following sample results are possible:
| |
Ct value |
Result Analysis |
| 1# |
No Ct |
Negative |
| 2# |
≤38 |
Positive |
| 3# |
38~40 |
Re-test; if it is still 38~40, report as 2# |
Reaction mix preparation :
Refer to one of the table below to prepare the reaction mix:
| 1×volume required |
| Resuspended master mix |
14 μL |
| HPV Primer-Probe Mix |
1 μL |
| Total volume |
15 μL |
※Multiply the numbers according to the number of tests.
Interpretation of test results:
| |
FAM (HPV16) |
VIC/HEX (HPV18) |
ROX(IC) |
Interpretation test results |
| 1 |
- |
- |
- |
Result invalid, test again |
| 2 |
+ |
- |
+/- |
HPV16(+) |
| 3 |
- |
+ |
+/- |
HPV18(+) |
| 4 |
+ |
+ |
+/- |
HPV16 and HPV18(+) |
| 5 |
- |
- |
+ |
Negative(-) |
NOTE: ‘+ ’ is positive, ‘- ’ is negative.
Operation details please check from IFU
Product Tags:
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Real Time Hpv Real Time Pcr Kit High Risk Micgene Nucleic Acid DNA Detection Kit Images
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